Names & Taxonomy

Uniprot ID:
Q16740
Entry Name:
CLPP_HUMAN
Status:
reviewed
Protein Names:
ATP-dependent Clp protease proteolytic subunit, mitochondrial (EC 3.4.21.92) (Endopeptidase Clp)
Gene Names:
CLPP
Gene Names Primary:
CLPP
Organism:
Homo sapiens (Human)

Structure

Length:
277
Sequence:
MWPGILVGGARVASCRYPALGPRLAAHFPAQRPPQRTLQNGLALQRCLHATATRALPLIPIVVEQTGRGERAYDIYSRLLRERIVCVMGPIDDSVASLVIAQLLFLQSESNKKPIHMYINSPGGVVTAGLAIYDTMQYILNPICTWCVGQAASMGSLLLAAGTPGMRHSLPNSRIMIHQPSGGARGQATDIAIQAEEIMKLKKQLYNIYAKHTKQSLQVIESAMERDRYMSPMEAQEFGILDKVLVHPPQDGEDEPTLVQKEPVEAAPAAEPVPAST
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Mitochondrion matrix

Function

Function:
Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
Catalytic Activity:
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
Active Site:
ACT_SITE 153 153 Nucleophile.
Gene Ontology Go:
endopeptidase Clp complex
mitochondrial matrix
mitochondrion
peptidase activity
serine-type endopeptidase activity
protein homooligomerization
proteolysis involved in cellular protein catabolic process
Gene Ontology Biological Process:
protein homooligomerization
proteolysis involved in cellular protein catabolic process
Gene Ontology Molecular Function:
peptidase activity
serine-type endopeptidase activity
Gene Ontology Cellular Component:
endopeptidase Clp complex
mitochondrial matrix
mitochondrion
Keywords:
3D-structure
Acetylation
Complete proteome
Deafness
Direct protein sequencing
Disease mutation
Hydrolase
Mitochondrion
Protease
Reference proteome
Serine protease
Transit peptide

Publication

PubMed ID:
8543061 14702039 15489334 19892738 10525407 11923310 16115876 19413330 19608861 21269460 24275569 25944712 15522782 23541340 25956234