Names & Taxonomy
- Uniprot ID:
- Q04760
- Entry Name:
- LGUL_HUMAN
- Status:
- reviewed
- Protein Names:
- Lactoylglutathione lyase (EC 4.4.1.5) (Aldoketomutase) (Glyoxalase I) (Glx I) (Ketone-aldehyde mutase) (Methylglyoxalase) (S-D-lactoylglutathione methylglyoxal lyase)
- Gene Names:
- GLO1
- Gene Names Primary:
- GLO1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 184
- Sequence:
- MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
- Proteomes:
- UP000005640
Function
- Function:
- Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.
- Pathway:
- Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
- Catalytic Activity:
- (R)-S-lactoylglutathione = glutathione + methylglyoxal.
- Cofactor:
- COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: ; Kinetic parameters: KM=1.3 mM for methylglyoxal/glutathione (native form)
- Enzyme Regulation:
- ENZYME REGULATION: Regulated by oxidation of Cys-139 in response to the redox state of the cell. Results in the alternative formation of cystine or glutathione-bound cysteine, the latter modification leading to reduced enzyme activity.
- Active Site:
- ACT_SITE 173 173 Proton donor/acceptor.
- Cross Reference Drug Bank:
- DB00143 DB00328
- Gene Ontology Go:
- cytoplasm
cytosol
extracellular exosome
lactoylglutathione lyase activity
zinc ion binding
carbohydrate metabolic process
cellular metabolic process
glutathione metabolic process
methylglyoxal metabolic process
negative regulation of apoptotic process
osteoclast differentiation
pyruvate metabolic process
regulation of transcription from RNA polymerase II promoter
small molecule metabolic process - Gene Ontology Biological Process:
- carbohydrate metabolic process
cellular metabolic process
glutathione metabolic process
methylglyoxal metabolic process
negative regulation of apoptotic process
osteoclast differentiation
pyruvate metabolic process
regulation of transcription from RNA polymerase II promoter
small molecule metabolic process - Gene Ontology Molecular Function:
- lactoylglutathione lyase activity
zinc ion binding - Gene Ontology Cellular Component:
- cytoplasm
cytosol
extracellular exosome - Keywords:
- 3D-structure
Acetylation
Alternative splicing
Complete proteome
Direct protein sequencing
Disulfide bond
Glutathionylation
Lyase
Metal-binding
Phosphoprotein
Polymorphism
Reference proteome
Zinc