Names & Taxonomy
- Uniprot ID:
- Q04609
- Entry Name:
- FOLH1_HUMAN
- Status:
- reviewed
- Protein Names:
- Glutamate carboxypeptidase 2 (EC 3.4.17.21) (Cell growth-inhibiting gene 27 protein) (Folate hydrolase 1) (Folylpoly-gamma-glutamate carboxypeptidase) (FGCP) (Glutamate carboxypeptidase II) (GCPII) (Membrane glutamate carboxypeptidase) (mGCP) (N-acetylated-alpha-linked acidic dipeptidase I) (NAALADase I) (Prostate-specific membrane antigen) (PSM) (PSMA) (Pteroylpoly-gamma-glutamate carboxypeptidase)
- Gene Names:
- FOLH1 FOLH NAALAD1 PSM PSMA GIG27
- Gene Names Orf:
- GIG27
- Gene Names Primary:
- FOLH1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 750
- Sequence:
- MWNLLHETDSAVATARRPRWLCAGALVLAGGFFLLGFLFGWFIKSSNEATNITPKHNMKAFLDELKAENIKKFLYNFTQIPHLAGTEQNFQLAKQIQSQWKEFGLDSVELAHYDVLLSYPNKTHPNYISIINEDGNEIFNTSLFEPPPPGYENVSDIVPPFSAFSPQGMPEGDLVYVNYARTEDFFKLERDMKINCSGKIVIARYGKVFRGNKVKNAQLAGAKGVILYSDPADYFAPGVKSYPDGWNLPGGGVQRGNILNLNGAGDPLTPGYPANEYAYRRGIAEAVGLPSIPVHPIGYYDAQKLLEKMGGSAPPDSSWRGSLKVPYNVGPGFTGNFSTQKVKMHIHSTNEVTRIYNVIGTLRGAVEPDRYVILGGHRDSWVFGGIDPQSGAAVVHEIVRSFGTLKKEGWRPRRTILFASWDAEEFGLLGSTEWAEENSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVHNLTKELKSPDEGFEGKSLYESWTKKSPSPEFSGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWETNKFSGYPLYHSVYETYELVEKFYDPMFKYHLTVAQVRGGMVFELANSIVLPFDCRDYAVVLRKYADKIYSISMKHPQEMKTYSVSFDSLFSAVKNFTEIASKFSERLQDFDKSNPIVLRMMNDQLMFLERAFIDPLGLPDRPFYRHVIYAPSSHNKYAGESFPGIYDALFDIESKVDPSKAWGEVKRQIYVAAFTVQAAAETLSEVA
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cell membrane
Function
- Function:
- Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.; Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
- Catalytic Activity:
- Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.
- Cofactor:
- COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
- Enzyme Regulation:
- ENZYME REGULATION: The NAALADase activity is inhibited by beta-NAAG, quisqualic acid, 2-(phosphonomethyl) pentanedioic acid (PMPA) and EDTA. Activated by cobalt.
- Active Site:
- ACT_SITE 424 424 Nucleophile; for NAALADase activity.; ACT_SITE 628 628 Charge relay system.
- Cross Reference Drug Bank:
- DB00089
- Gene Ontology Go:
- cell surface
cytoplasm
extracellular exosome
integral component of plasma membrane
membrane
plasma membrane
Ac-Asp-Glu binding
dipeptidase activity
metal ion binding
metallocarboxypeptidase activity
peptidase activity
tetrahydrofolyl-poly(glutamate) polymer binding
C-terminal protein deglutamylation
cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
folic acid-containing compound metabolic process
proteolysis
small molecule metabolic process - Gene Ontology Biological Process:
- cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
C-terminal protein deglutamylation
folic acid-containing compound metabolic process
proteolysis
small molecule metabolic process - Gene Ontology Molecular Function:
- Ac-Asp-Glu binding
dipeptidase activity
metal ion binding
metallocarboxypeptidase activity
peptidase activity
tetrahydrofolyl-poly(glutamate) polymer binding - Gene Ontology Cellular Component:
- cell surface
cytoplasm
extracellular exosome
integral component of plasma membrane
membrane
plasma membrane - Keywords:
- 3D-structure
Alternative splicing
Calcium
Carboxypeptidase
Cell membrane
Complete proteome
Cytoplasm
Dipeptidase
Direct protein sequencing
Glycoprotein
Hydrolase
Membrane
Metal-binding
Metalloprotease
Multifunctional enzyme
Phosphoprotein
Polymorphism
Protease
Reference proteome
Signal-anchor
Transmembrane
Transmembrane helix
Zinc