Names & Taxonomy
- Uniprot ID:
- P78329
- Entry Name:
- CP4F2_HUMAN
- Status:
- reviewed
- Protein Names:
- Phylloquinone omega-hydroxylase CYP4F2 (EC 1.14.13.194) (20-hydroxyeicosatetraenoic acid synthase) (20-HETE synthase) (EC 1.14.13.-) (Arachidonic acid omega-hydroxylase) (CYPIVF2) (Cytochrome P450 4F2) (Cytochrome P450-LTB-omega) (Leukotriene-B(4) 20-monooxygenase 1) (Leukotriene-B(4) omega-hydroxylase 1) (EC 1.14.13.30)
- Gene Names:
- CYP4F2
- Gene Names Primary:
- CYP4F2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 520
- Sequence:
- MSQLSLSWLGLWPVAASPWLLLLLVGASWLLAHVLAWTYAFYDNCRRLRCFPQPPRRNWFWGHQGMVNPTEEGMRVLTQLVATYPQGFKVWMGPISPLLSLCHPDIIRSVINASAAIAPKDKFFYSFLEPWLGDGLLLSAGDKWSRHRRMLTPAFHFNILKPYMKIFNESVNIMHAKWQLLASEGSACLDMFEHISLMTLDSLQKCVFSFDSHCQEKPSEYIAAILELSALVSKRHHEILLHIDFLYYLTPDGQRFRRACRLVHDFTDAVIQERRRTLPSQGVDDFLQAKAKSKTLDFIDVLLLSKDEDGKKLSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEYQERCRQEVQELLKDREPKEIEWDDLAHLPFLTMCMKESLRLHPPVPVISRHVTQDIVLPDGRVIPKGIICLISVFGTHHNPAVWPDPEVYDPFRFDPENIKERSPLAFIPFSAGPRNCIGQTFAMAEMKVVLALTLLRFRVLPDHTEPRRKPELVLRAEGGLWLRVEPLS
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Microsome membrane
Function
- Function:
- Omega-hydroxylase that oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids and xenobiotics. Plays a key role in vitamin K catabolism by mediating omega-hydroxylation of vitamin K1 (phylloquinone), and menaquinone-4 (MK-4), a form of vitamin K2. Hydroxylation of phylloquinone and MK-4 probably regulates blood coagulation (PubMed:19297519, PubMed:24138531). Also shows arachidonic acid omega-hydroxylase activity in kidney, by mediating conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), possibly influencing blood pressure control (PubMed:10660572, PubMed:17341693, PubMed:18574070). Also acts as a leukotriene-B(4) omega-hydroxylase by mediating conversion of leukotriene-B(4) (LTB4) to its omega-hydroxylated metabolite 20-hydroxyleukotriene-B(4) (20-OH LTB4) (PubMed:8026587, PubMed:9799565).
- Pathway:
- Cofactor degradation; phylloquinone degradation.
- Catalytic Activity:
- (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O.
- Cofactor:
- COFACTOR: Name=heme; Xref=ChEBI:CHEBI:30413;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: ; Kinetic parameters: KM=74.8 uM for leukotriene-B(4)
- Cross Reference Drug Bank:
- DB08868
- Gene Ontology Go:
- apical plasma membrane
cytoplasm
endoplasmic reticulum membrane
intracellular membrane-bounded organelle
alkane 1-monooxygenase activity
alpha-tocopherol omega-hydroxylase activity
arachidonic acid epoxygenase activity
arachidonic acid omega-hydroxylase activity
heme binding
iron ion binding
leukotriene-B4 20-monooxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
tocotrienol omega-hydroxylase activity
arachidonic acid metabolic process
blood coagulation
drug metabolic process
epoxygenase P450 pathway
icosanoid metabolic process
leukotriene B4 catabolic process
leukotriene metabolic process
long-chain fatty acid metabolic process
menaquinone catabolic process
negative regulation of icosanoid secretion
omega-hydroxylase P450 pathway
oxidation-reduction process
phylloquinone catabolic process
positive regulation of icosanoid secretion
pressure natriuresis
regulation of blood pressure
renal water homeostasis
small molecule metabolic process
sodium ion homeostasis
very long-chain fatty acid metabolic process
vitamin E metabolic process
vitamin K catabolic process
xenobiotic metabolic process - Gene Ontology Biological Process:
- arachidonic acid metabolic process
blood coagulation
drug metabolic process
epoxygenase P450 pathway
icosanoid metabolic process
leukotriene B4 catabolic process
leukotriene metabolic process
long-chain fatty acid metabolic process
menaquinone catabolic process
negative regulation of icosanoid secretion
omega-hydroxylase P450 pathway
oxidation-reduction process
phylloquinone catabolic process
positive regulation of icosanoid secretion
pressure natriuresis
regulation of blood pressure
renal water homeostasis
small molecule metabolic process
sodium ion homeostasis
very long-chain fatty acid metabolic process
vitamin E metabolic process
vitamin K catabolic process
xenobiotic metabolic process - Gene Ontology Molecular Function:
- alkane 1-monooxygenase activity
alpha-tocopherol omega-hydroxylase activity
arachidonic acid epoxygenase activity
arachidonic acid omega-hydroxylase activity
heme binding
iron ion binding
leukotriene-B4 20-monooxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
tocotrienol omega-hydroxylase activity - Gene Ontology Cellular Component:
- apical plasma membrane
cytoplasm
endoplasmic reticulum membrane
intracellular membrane-bounded organelle - Keywords:
- Alternative splicing
Complete proteome
Direct protein sequencing
Endoplasmic reticulum
Heme
Iron
Membrane
Metal-binding
Microsome
Monooxygenase
NADP
Oxidoreductase
Polymorphism
Reference proteome - Interacts With:
- P16333