Names & Taxonomy
- Uniprot ID:
- P49917
- Entry Name:
- DNLI4_HUMAN
- Status:
- reviewed
- Protein Names:
- DNA ligase 4 (EC 6.5.1.1) (DNA ligase IV) (Polydeoxyribonucleotide synthase [ATP] 4)
- Gene Names:
- LIG4
- Gene Names Primary:
- LIG4
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 911
- Sequence:
- MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNEAIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus.
Function
- Function:
- Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
- Catalytic Activity:
- ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Active Site:
- ACT_SITE 273 273 N6-AMP-lysine intermediate.
- Gene Ontology Go:
- condensed chromosome
cytoplasm
DNA ligase IV complex
DNA-dependent protein kinase-DNA ligase 4 complex
focal adhesion
nonhomologous end joining complex
nuclear chromosome, telomeric region
nucleoplasm
nucleus
plasma membrane
ATP binding
DNA binding
DNA ligase (ATP) activity
DNA ligase activity
ligase activity
metal ion binding
protein C-terminus binding
cell cycle
cell division
cell proliferation
cellular response to lithium ion
central nervous system development
chromosome organization
DNA biosynthetic process
DNA ligation
DNA ligation involved in DNA recombination
DNA ligation involved in DNA repair
DNA repair
DNA replication
double-strand break repair
double-strand break repair via classical nonhomologous end joining
double-strand break repair via nonhomologous end joining
establishment of integrated proviral latency
immunoglobulin V(D)J recombination
in utero embryonic development
isotype switching
negative regulation of neuron apoptotic process
neuron apoptotic process
nucleotide-excision repair, DNA gap filling
positive regulation of chromosome organization
positive regulation of fibroblast proliferation
positive regulation of neurogenesis
pro-B cell differentiation
response to gamma radiation
response to X-ray
single strand break repair
somatic stem cell population maintenance
T cell differentiation in thymus
T cell receptor V(D)J recombination
V(D)J recombination
viral process - Gene Ontology Biological Process:
- cell cycle
cell division
cell proliferation
cellular response to lithium ion
central nervous system development
chromosome organization
DNA biosynthetic process
DNA ligation
DNA ligation involved in DNA recombination
DNA ligation involved in DNA repair
DNA repair
DNA replication
double-strand break repair
double-strand break repair via classical nonhomologous end joining
double-strand break repair via nonhomologous end joining
establishment of integrated proviral latency
immunoglobulin V(D)J recombination
in utero embryonic development
isotype switching
negative regulation of neuron apoptotic process
neuron apoptotic process
nucleotide-excision repair, DNA gap filling
positive regulation of chromosome organization
positive regulation of fibroblast proliferation
positive regulation of neurogenesis
pro-B cell differentiation
response to gamma radiation
response to X-ray
single strand break repair
somatic stem cell population maintenance
T cell differentiation in thymus
T cell receptor V(D)J recombination
V(D)J recombination
viral process - Gene Ontology Molecular Function:
- ATP binding
DNA binding
DNA ligase (ATP) activity
DNA ligase activity
ligase activity
metal ion binding
protein C-terminus binding - Gene Ontology Cellular Component:
- condensed chromosome
cytoplasm
DNA-dependent protein kinase-DNA ligase 4 complex
DNA ligase IV complex
focal adhesion
nonhomologous end joining complex
nuclear chromosome, telomeric region
nucleoplasm
nucleus
plasma membrane - Keywords:
- 3D-structure
ATP-binding
Cell cycle
Cell division
Complete proteome
DNA damage
DNA recombination
DNA repair
DNA replication
Direct protein sequencing
Disease mutation
Ligase
Magnesium
Metal-binding
Nucleotide-binding
Nucleus
Polymorphism
Reference proteome
Repeat
SCID - Interacts With:
- Q8IW19; Q9H9Q4; Q13426