Names & Taxonomy

Uniprot ID:
P49916
Entry Name:
DNLI3_HUMAN
Status:
reviewed
Protein Names:
DNA ligase 3 (EC 6.5.1.1) (DNA ligase III) (Polydeoxyribonucleotide synthase [ATP] 3)
Gene Names:
LIG3
Gene Names Primary:
LIG3
Organism:
Homo sapiens (Human)

Structure

Length:
1009
Sequence:
MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Isoform 1: Mitochondrion

Function

Function:
Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627).
Catalytic Activity:
ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor:
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Active Site:
ACT_SITE 508 508 N6-AMP-lysine intermediate.
Cross Reference Drug Bank:
DB00290
Gene Ontology Go:
DNA ligase III-XRCC1 complex
mitochondrion
nucleoplasm
nucleus
ATP binding
DNA binding
DNA ligase (ATP) activity
DNA ligase activity
zinc ion binding
base-excision repair
base-excision repair, DNA ligation
cell cycle
cell division
DNA biosynthetic process
DNA ligation
DNA repair
DNA replication
double-strand break repair
double-strand break repair via alternative nonhomologous end joining
double-strand break repair via homologous recombination
global genome nucleotide-excision repair
mitochondrial DNA repair
mitochondrion organization
negative regulation of mitochondrial DNA replication
nucleotide-excision repair
nucleotide-excision repair, DNA gap filling
transcription-coupled nucleotide-excision repair
Gene Ontology Biological Process:
base-excision repair
base-excision repair, DNA ligation
cell cycle
cell division
DNA biosynthetic process
DNA ligation
DNA repair
DNA replication
double-strand break repair
double-strand break repair via alternative nonhomologous end joining
double-strand break repair via homologous recombination
global genome nucleotide-excision repair
mitochondrial DNA repair
mitochondrion organization
negative regulation of mitochondrial DNA replication
nucleotide-excision repair
nucleotide-excision repair, DNA gap filling
transcription-coupled nucleotide-excision repair
Gene Ontology Molecular Function:
ATP binding
DNA binding
DNA ligase (ATP) activity
DNA ligase activity
zinc ion binding
Gene Ontology Cellular Component:
DNA ligase III-XRCC1 complex
mitochondrion
nucleoplasm
nucleus
Keywords:
3D-structure
ATP-binding
Alternative initiation
Alternative splicing
Cell cycle
Cell division
Complete proteome
DNA damage
DNA recombination
DNA repair
DNA replication
Ligase
Magnesium
Metal-binding
Mitochondrion
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Transit peptide
Zinc
Zinc-finger
Interacts With:
P61244

Publication

PubMed ID:
7760816 7565692 20843780 16625196 15489334 10207110 17081983 16964243 18669648 19413330 19690332 20068231 21269460 21406692 24674627 11281714 15288782 20518483 21652643 16959974