Names & Taxonomy
- Uniprot ID:
- P49916
- Entry Name:
- DNLI3_HUMAN
- Status:
- reviewed
- Protein Names:
- DNA ligase 3 (EC 6.5.1.1) (DNA ligase III) (Polydeoxyribonucleotide synthase [ATP] 3)
- Gene Names:
- LIG3
- Gene Names Primary:
- LIG3
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1009
- Sequence:
- MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Isoform 1: Mitochondrion
Function
- Function:
- Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627).
- Catalytic Activity:
- ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Active Site:
- ACT_SITE 508 508 N6-AMP-lysine intermediate.
- Cross Reference Drug Bank:
- DB00290
- Gene Ontology Go:
- DNA ligase III-XRCC1 complex
mitochondrion
nucleoplasm
nucleus
ATP binding
DNA binding
DNA ligase (ATP) activity
DNA ligase activity
zinc ion binding
base-excision repair
base-excision repair, DNA ligation
cell cycle
cell division
DNA biosynthetic process
DNA ligation
DNA repair
DNA replication
double-strand break repair
double-strand break repair via alternative nonhomologous end joining
double-strand break repair via homologous recombination
global genome nucleotide-excision repair
mitochondrial DNA repair
mitochondrion organization
negative regulation of mitochondrial DNA replication
nucleotide-excision repair
nucleotide-excision repair, DNA gap filling
transcription-coupled nucleotide-excision repair - Gene Ontology Biological Process:
- base-excision repair
base-excision repair, DNA ligation
cell cycle
cell division
DNA biosynthetic process
DNA ligation
DNA repair
DNA replication
double-strand break repair
double-strand break repair via alternative nonhomologous end joining
double-strand break repair via homologous recombination
global genome nucleotide-excision repair
mitochondrial DNA repair
mitochondrion organization
negative regulation of mitochondrial DNA replication
nucleotide-excision repair
nucleotide-excision repair, DNA gap filling
transcription-coupled nucleotide-excision repair - Gene Ontology Molecular Function:
- ATP binding
DNA binding
DNA ligase (ATP) activity
DNA ligase activity
zinc ion binding - Gene Ontology Cellular Component:
- DNA ligase III-XRCC1 complex
mitochondrion
nucleoplasm
nucleus - Keywords:
- 3D-structure
ATP-binding
Alternative initiation
Alternative splicing
Cell cycle
Cell division
Complete proteome
DNA damage
DNA recombination
DNA repair
DNA replication
Ligase
Magnesium
Metal-binding
Mitochondrion
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Transit peptide
Zinc
Zinc-finger - Interacts With:
- P61244