Names & Taxonomy

Uniprot ID:
P45452
Entry Name:
MMP13_HUMAN
Status:
reviewed
Protein Names:
Collagenase 3 (EC 3.4.24.-) (Matrix metalloproteinase-13) (MMP-13)
Gene Names:
MMP13
Gene Names Primary:
MMP13
Organism:
Homo sapiens (Human)

Structure

Length:
471
Sequence:
MHPGVLAAFLFLSWTHCRALPLPSGGDEDDLSEEDLQFAERYLRSYYHPTNLAGILKENAASSMTERLREMQSFFGLEVTGKLDDNTLDVMKKPRCGVPDVGEYNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Secreted, extracellular space, extracellular matrix

Function

Function:
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Cofactor:
COFACTOR: Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
Enzyme Regulation:
ENZYME REGULATION: Inhibited by TIMP1, TIMP2 and TIMP3. Inhibited by acetohydroxamic acid and other zinc chelators.
Active Site:
ACT_SITE 223 223
Cross Reference Drug Bank:
DB00786
Gene Ontology Go:
extracellular region
extracellular space
Golgi apparatus
intercellular canaliculus
lysosome
proteinaceous extracellular matrix
calcium ion binding
collagen binding
metalloendopeptidase activity
zinc ion binding
bone mineralization
bone morphogenesis
cellular protein metabolic process
cellular response to fluid shear stress
collagen catabolic process
embryonic hindlimb morphogenesis
endochondral ossification
estrous cycle
extracellular matrix disassembly
extracellular matrix organization
growth plate cartilage development
heart development
luteolysis
osteoblast differentiation
parturition
peptide catabolic process
positive regulation of pancreatic trypsinogen secretion
proteolysis
response to drug
response to estrogen
response to hypoxia
response to mechanical stimulus
Gene Ontology Biological Process:
bone mineralization
bone morphogenesis
cellular protein metabolic process
cellular response to fluid shear stress
collagen catabolic process
embryonic hindlimb morphogenesis
endochondral ossification
estrous cycle
extracellular matrix disassembly
extracellular matrix organization
growth plate cartilage development
heart development
luteolysis
osteoblast differentiation
parturition
peptide catabolic process
positive regulation of pancreatic trypsinogen secretion
proteolysis
response to drug
response to estrogen
response to hypoxia
response to mechanical stimulus
Gene Ontology Molecular Function:
calcium ion binding
collagen binding
metalloendopeptidase activity
zinc ion binding
Gene Ontology Cellular Component:
extracellular region
extracellular space
Golgi apparatus
intercellular canaliculus
lysosome
proteinaceous extracellular matrix
Keywords:
3D-structure
Calcium
Collagen degradation
Complete proteome
Direct protein sequencing
Disease mutation
Disulfide bond
Dwarfism
Extracellular matrix
Glycoprotein
Hydrolase
Metal-binding
Metalloprotease
Phosphoprotein
Polymorphism
Protease
Reference proteome
Repeat
Secreted
Signal
Zinc
Zymogen

Publication

PubMed ID:
8207000 9562863 14702039 15489334 8576151 8663255 8603731 8798568 9056642 9065415 24648384 25171405 24781753 8969305 10074939 10926524 10986126 15780611 15734645 17623656 17196980 19422229 20005097 20726512 22689580 23810497 23913860 16167086 19615667