Names & Taxonomy
- Uniprot ID:
- P30291
- Entry Name:
- WEE1_HUMAN
- Status:
- reviewed
- Protein Names:
- Wee1-like protein kinase (WEE1hu) (EC 2.7.10.2) (Wee1A kinase)
- Gene Names:
- WEE1
- Gene Names Primary:
- WEE1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 646
- Sequence:
- MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEEEEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTEPGPERRRSPGPAPGSPGELEEDLLLPGACPGADEAGGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSPVRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPPHKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRGSSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLLHSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHSHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSAMALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus.
Function
- Function:
- Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
- Catalytic Activity:
- ATP + a -L-tyrosine = ADP + a -L-tyrosine phosphate.
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; ; Note=Binds 2 magnesium ions per subunit.;
- Enzyme Regulation:
- ENZYME REGULATION: Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.
- Active Site:
- ACT_SITE 426 426 Proton acceptor.
- Gene Ontology Go:
- cytoplasm
nucleolus
nucleoplasm
nucleus
ATP binding
magnesium ion binding
non-membrane spanning protein tyrosine kinase activity
protein tyrosine kinase activity
blood coagulation
cell division
establishment of cell polarity
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
microtubule cytoskeleton organization
mitotic cell cycle
mitotic nuclear division
neuron projection morphogenesis
regulation of cell cycle - Gene Ontology Biological Process:
- blood coagulation
cell division
establishment of cell polarity
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
microtubule cytoskeleton organization
mitotic cell cycle
mitotic nuclear division
neuron projection morphogenesis
regulation of cell cycle - Gene Ontology Molecular Function:
- ATP binding
magnesium ion binding
non-membrane spanning protein tyrosine kinase activity
protein tyrosine kinase activity - Gene Ontology Cellular Component:
- cytoplasm
nucleolus
nucleoplasm
nucleus - Keywords:
- 3D-structure
ATP-binding
Alternative splicing
Cell cycle
Cell division
Complete proteome
Kinase
Magnesium
Metal-binding
Mitosis
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Transferase
Tyrosine-protein kinase
Ubl conjugation - Interacts With:
- Q9Y297; Q9UKB1; P63104