Names & Taxonomy
- Uniprot ID:
- P28838
- Entry Name:
- AMPL_HUMAN
- Status:
- reviewed
- Protein Names:
- Cytosol aminopeptidase (EC 3.4.11.1) (Leucine aminopeptidase 3) (LAP-3) (Leucyl aminopeptidase) (Peptidase S) (Proline aminopeptidase) (EC 3.4.11.5) (Prolyl aminopeptidase)
- Gene Names:
- LAP3 LAPEP PEPS
- Gene Names Primary:
- LAP3
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 519
- Sequence:
- MFLLPLPAAGRVVVRRLAVRRFGSRSLSTADMTKGLVLGIYSKEKEDDVPQFTSAGENFDKLLAGKLRETLNISGPPLKAGKTRTFYGLHQDFPSVVLVGLGKKAAGIDEQENWHEGKENIRAAVAAGCRQIQDLELSSVEVDPCGDAQAAAEGAVLGLYEYDDLKQKKKMAVSAKLYGSGDQEAWQKGVLFASGQNLARQLMETPANEMTPTRFAEIIEKNLKSASSKTEVHIRPKSWIEEQAMGSFLSVAKGSDEPPVFLEIHYKGSPNANEPPLVFVGKGITFDSGGISIKASANMDLMRADMGGAATICSAIVSAAKLNLPINIIGLAPLCENMPSGKANKPGDVVRAKNGKTIQVDNTDAEGRLILADALCYAHTFNPKVILNAATLTGAMDVALGSGATGVFTNSSWLWNKLFEASIETGDRVWRMPLFEHYTRQVVDCQLADVNNIGKYRSAGACTAAAFLKEFVTHPKWAHLDIAGVMTNKDEVPYLRKGMTGRPTRTLIEFLLRFSQDNA
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm.
Function
- Function:
- Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.
- Catalytic Activity:
- Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.; Release of N-terminal proline from a peptide.
- Cofactor:
- COFACTOR: ; Note=Binds 2 zinc ions per subunit. One zinc ion is tightly bound and essential for enzyme activity, while the second metal coordination site can be occupied by zinc, magnesium or manganese to give enzymes of different activities.
- Active Site:
- ACT_SITE 294 294
- Gene Ontology Go:
- cytoplasm
extracellular exosome
focal adhesion
mitochondrion
nucleoplasm
nucleus
trans-Golgi network
aminopeptidase activity
manganese ion binding
metalloexopeptidase activity
proteolysis - Gene Ontology Biological Process:
- proteolysis
- Gene Ontology Molecular Function:
- aminopeptidase activity
manganese ion binding
metalloexopeptidase activity - Gene Ontology Cellular Component:
- cytoplasm
extracellular exosome
focal adhesion
mitochondrion
nucleoplasm
nucleus
trans-Golgi network - Keywords:
- Acetylation
Alternative initiation
Aminopeptidase
Complete proteome
Cytoplasm
Direct protein sequencing
Hydrolase
Magnesium
Manganese
Metal-binding
Phosphoprotein
Protease
Reference proteome
Zinc