Names & Taxonomy

Uniprot ID:
P24752
Entry Name:
THIL_HUMAN
Status:
reviewed
Protein Names:
Acetyl-CoA acetyltransferase, mitochondrial (EC 2.3.1.9) (Acetoacetyl-CoA thiolase) (T2)
Gene Names:
ACAT1 ACAT MAT
Gene Names Primary:
ACAT1
Organism:
Homo sapiens (Human)

Structure

Length:
427
Sequence:
MAVLAALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVMNRGSTPYGGVKLEDLIVKDGLTDVYNKIHMGSCAENTAKKLNIARNEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLEIDPQKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQKL
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Mitochondrion.

Function

Function:
Plays a major role in ketone body metabolism.
Catalytic Activity:
2 acetyl-CoA = CoA + acetoacetyl-CoA.
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=4 uM for acetoacetyl coenzyme A
Enzyme Regulation:
ENZYME REGULATION: Activated by potassium ions, but not sodium ions.
Active Site:
ACT_SITE 126 126 Acyl-thioester intermediate.
Cross Reference Drug Bank:
DB00795
Gene Ontology Go:
extracellular exosome
mitochondrial inner membrane
mitochondrial matrix
mitochondrion
acetyl-CoA C-acetyltransferase activity
coenzyme binding
metal ion binding
adipose tissue development
brain development
branched-chain amino acid catabolic process
cellular ketone body metabolic process
cellular lipid metabolic process
cellular nitrogen compound metabolic process
ketone body biosynthetic process
ketone body catabolic process
liver development
metanephric proximal convoluted tubule development
protein homooligomerization
response to hormone
response to organic cyclic compound
response to starvation
small molecule metabolic process
Gene Ontology Biological Process:
adipose tissue development
brain development
branched-chain amino acid catabolic process
cellular ketone body metabolic process
cellular lipid metabolic process
cellular nitrogen compound metabolic process
ketone body biosynthetic process
ketone body catabolic process
liver development
metanephric proximal convoluted tubule development
protein homooligomerization
response to hormone
response to organic cyclic compound
response to starvation
small molecule metabolic process
Gene Ontology Molecular Function:
acetyl-CoA C-acetyltransferase activity
coenzyme binding
metal ion binding
Gene Ontology Cellular Component:
extracellular exosome
mitochondrial inner membrane
mitochondrial matrix
mitochondrion
Keywords:
3D-structure
Acetylation
Acyltransferase
Alternative splicing
Complete proteome
Direct protein sequencing
Disease mutation
Metal-binding
Mitochondrion
Polymorphism
Potassium
Reference proteome
Transferase
Transit peptide

Publication

PubMed ID:
1979337 1684944 14702039 16554811 15489334 15242332 18669648 19608861 21269460 24275569 25944712 17371050 7749408 1346617 1715688 7728148 9744475