Names & Taxonomy
- Uniprot ID:
- P23141
- Entry Name:
- EST1_HUMAN
- Status:
- reviewed
- Protein Names:
- Liver carboxylesterase 1 (Acyl-coenzyme A:cholesterol acyltransferase) (ACAT) (Brain carboxylesterase hBr1) (Carboxylesterase 1) (CE-1) (hCE-1) (EC 3.1.1.1) (Cocaine carboxylesterase) (Egasyn) (HMSE) (Methylumbelliferyl-acetate deacetylase 1) (EC 3.1.1.56) (Monocyte/macrophage serine esterase) (Retinyl ester hydrolase) (REH) (Serine esterase 1) (Triacylglycerol hydrolase) (TGH)
- Gene Names:
- CES1 CES2 SES1
- Gene Names Primary:
- CES1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 567
- Sequence:
- MWLRAFILATLSASAAWGHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMQLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Endoplasmic reticulum lumen
Function
- Function:
- Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
- Catalytic Activity:
- A carboxylic ester + H(2)O = an alcohol + a carboxylate.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=106.6 uM for p-nitrophenyl acetate
- Enzyme Regulation:
- ENZYME REGULATION: Activated by CHAPS.
- Active Site:
- ACT_SITE 221 221 Acyl-ester intermediate.
- Cross Reference Drug Bank:
- DB01086 DB01101 DB02659 DB01410 DB00758 DB04838 DB06695 DB00328 DB00762 DB00583 DB00688 DB00198 DB01599 DB06201 DB11362 DB00675 DB00519
- Gene Ontology Go:
- endoplasmic reticulum lumen
extracellular space
carboxylic ester hydrolase activity
methylumbelliferyl-acetate deacetylase activity
epithelial cell differentiation
metabolic process
response to toxic substance
small molecule metabolic process
xenobiotic metabolic process - Gene Ontology Biological Process:
- epithelial cell differentiation
metabolic process
response to toxic substance
small molecule metabolic process
xenobiotic metabolic process - Gene Ontology Molecular Function:
- carboxylic ester hydrolase activity
methylumbelliferyl-acetate deacetylase activity - Gene Ontology Cellular Component:
- endoplasmic reticulum lumen
extracellular space - Keywords:
- 3D-structure
Alternative splicing
Complete proteome
Direct protein sequencing
Disulfide bond
Endoplasmic reticulum
Glycoprotein
Hydrolase
Phosphoprotein
Polymorphism
Reference proteome
Serine esterase
Signal