Names & Taxonomy

Uniprot ID:
P22748
Entry Name:
CAH4_HUMAN
Status:
reviewed
Protein Names:
Carbonic anhydrase 4 (EC 4.2.1.1) (Carbonate dehydratase IV) (Carbonic anhydrase IV) (CA-IV)
Gene Names:
CA4
Gene Names Primary:
CA4
Organism:
Homo sapiens (Human)

Structure

Length:
312
Sequence:
MRMLLALLALSAARPSASAESHWCYEVQAESSNYPCLVPVKWGGNCQKDRQSPINIVTTKAKVDKKLGRFFFSGYDKKQTWTVQNNGHSVMMLLENKASISGGGLPAPYQAKQLHLHWSDLPYKGSEHSLDGEHFAMEMHIVHEKEKGTSRNVKEAQDPEDEIAVLAFLVEAGTQVNEGFQPLVEALSNIPKPEMSTTMAESSLLDLLPKEEKLRHYFRYLGSLTTPTCDEKVVWTVFREPIQLHREQILAFSQKLYYDKEQTVSMKDNVRPLQQLGQRTVIKSGAPGRPLPWALPALLGPMLACLLAGFLR
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Function

Function:
Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.
Catalytic Activity:
H(2)CO(3) = CO(2) + H(2)O.
Cofactor:
COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=21.5 mM for CO(2)
Enzyme Regulation:
ENZYME REGULATION: Activated by histamine, L-adrenaline, D-phenylalanine, L- and D-histidine. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).
Active Site:
ACT_SITE 88 88 Proton acceptor.
Cross Reference Drug Bank:
DB00819 DB00436 DB00562 DB01194 DB00880 DB00606 DB01144 DB00869 DB00999 DB00774 DB00703 DB00232 DB00273 DB01021 DB00909
Gene Ontology Go:
anchored component of external side of plasma membrane
apical plasma membrane
brush border membrane
cell surface
endoplasmic reticulum-Golgi intermediate compartment
extracellular exosome
Golgi apparatus
membrane
perinuclear region of cytoplasm
plasma membrane
rough endoplasmic reticulum
secretory granule membrane
trans-Golgi network
transport vesicle membrane
carbonate dehydratase activity
zinc ion binding
bicarbonate transport
one-carbon metabolic process
small molecule metabolic process
Gene Ontology Biological Process:
bicarbonate transport
one-carbon metabolic process
small molecule metabolic process
Gene Ontology Molecular Function:
carbonate dehydratase activity
zinc ion binding
Gene Ontology Cellular Component:
anchored component of external side of plasma membrane
apical plasma membrane
brush border membrane
cell surface
endoplasmic reticulum-Golgi intermediate compartment
extracellular exosome
Golgi apparatus
membrane
perinuclear region of cytoplasm
plasma membrane
rough endoplasmic reticulum
secretory granule membrane
trans-Golgi network
transport vesicle membrane
Keywords:
3D-structure
Alternative splicing
Cell membrane
Complete proteome
Direct protein sequencing
Disease mutation
Disulfide bond
GPI-anchor
Glycoprotein
Lipoprotein
Lyase
Membrane
Metal-binding
Polymorphism
Reference proteome
Retinitis pigmentosa
Signal
Zinc

Publication

PubMed ID:
1311094 8325641 14702039 16625196 15489334 2111324 8809084 7625839 16807956 16686544 17705204 17127057 17314045 19186056 19206230 18618712 8942978 20363633 15563508 17652713 20450258