Names & Taxonomy
- Uniprot ID:
- P16455
- Entry Name:
- MGMT_HUMAN
- Status:
- reviewed
- Protein Names:
- Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase)
- Gene Names:
- MGMT
- Gene Names Primary:
- MGMT
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 207
- Sequence:
- MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus.
Function
- Function:
- Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
- Catalytic Activity:
- DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
- Cofactor:
- COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; ; Note=Binds 1 zinc ion.;
- Active Site:
- ACT_SITE 145 145 Nucleophile; methyl group acceptor.
- Cross Reference Drug Bank:
- DB00151
- Gene Ontology Go:
- membrane
nucleoplasm
nucleus
calcium ion binding
damaged DNA binding
DNA binding
DNA-methyltransferase activity
methylated-DNA--cysteine S-methyltransferase activity
methyltransferase activity
cellular response to ionizing radiation
cellular response to organic cyclic compound
cellular response to oxidative stress
DNA dealkylation involved in DNA repair
DNA ligation
DNA methylation
DNA repair
mammary gland epithelial cell differentiation
negative regulation of apoptotic process
positive regulation of double-strand break repair
regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to drug
response to ethanol
response to folic acid
response to toxic substance - Gene Ontology Biological Process:
- cellular response to ionizing radiation
cellular response to organic cyclic compound
cellular response to oxidative stress
DNA dealkylation involved in DNA repair
DNA ligation
DNA methylation
DNA repair
mammary gland epithelial cell differentiation
negative regulation of apoptotic process
positive regulation of double-strand break repair
regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to drug
response to ethanol
response to folic acid
response to toxic substance - Gene Ontology Molecular Function:
- calcium ion binding
damaged DNA binding
DNA binding
DNA-methyltransferase activity
methylated-DNA--cysteine S-methyltransferase activity
methyltransferase activity - Gene Ontology Cellular Component:
- membrane
nucleoplasm
nucleus - Keywords:
- 3D-structure
Complete proteome
DNA damage
DNA repair
DNA-binding
Direct protein sequencing
Metal-binding
Methyltransferase
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Transferase
Zinc