Names & Taxonomy
- Uniprot ID:
- P15531
- Entry Name:
- NDKA_HUMAN
- Status:
- reviewed
- Protein Names:
- Nucleoside diphosphate kinase A (NDK A) (NDP kinase A) (EC 2.7.4.6) (Granzyme A-activated DNase) (GAAD) (Metastasis inhibition factor nm23) (NM23-H1) (Tumor metastatic process-associated protein)
- Gene Names:
- NME1 NDPKA NM23
- Gene Names Primary:
- NME1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 152
- Sequence:
- MANCERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIGLWFHPEELVDYTSCAQNWIYE
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm
Function
- Function:
- Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
- Catalytic Activity:
- ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Enzyme Regulation:
- ENZYME REGULATION: Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits the endonuclease activity.
- Active Site:
- ACT_SITE 118 118 Pros-phosphohistidine intermediate.
- Cross Reference Drug Bank:
- DB00718 DB00709 DB00300
- Gene Ontology Go:
- centrosome
cytoplasm
cytosol
extracellular exosome
intermediate filament
membrane
mitochondrial outer membrane
myelin sheath
nucleus
perinuclear region of cytoplasm
ruffle membrane
ATP binding
deoxyribonuclease activity
GTP binding
identical protein binding
magnesium ion binding
nucleoside diphosphate kinase activity
poly(A) RNA binding
ribosomal small subunit binding
RNA polymerase II regulatory region sequence-specific DNA binding
single-stranded DNA binding
cellular response to drug
cellular response to fatty acid
cellular response to glucose stimulus
CTP biosynthetic process
DNA metabolic process
endocytosis
GTP biosynthetic process
hippocampus development
lactation
negative regulation of cell proliferation
negative regulation of gene expression
negative regulation of myeloid leukocyte differentiation
nucleobase-containing small molecule interconversion
nucleobase-containing small molecule metabolic process
nucleoside triphosphate biosynthetic process
positive regulation of DNA binding
positive regulation of epithelial cell proliferation
positive regulation of neuron projection development
purine nucleotide metabolic process
pyrimidine nucleotide metabolic process
regulation of apoptotic process
response to amine
response to cAMP
response to testosterone
small molecule metabolic process
UTP biosynthetic process - Gene Ontology Biological Process:
- cellular response to drug
cellular response to fatty acid
cellular response to glucose stimulus
CTP biosynthetic process
DNA metabolic process
endocytosis
GTP biosynthetic process
hippocampus development
lactation
negative regulation of cell proliferation
negative regulation of gene expression
negative regulation of myeloid leukocyte differentiation
nucleobase-containing small molecule interconversion
nucleobase-containing small molecule metabolic process
nucleoside triphosphate biosynthetic process
positive regulation of DNA binding
positive regulation of epithelial cell proliferation
positive regulation of neuron projection development
purine nucleotide metabolic process
pyrimidine nucleotide metabolic process
regulation of apoptotic process
response to amine
response to cAMP
response to testosterone
small molecule metabolic process
UTP biosynthetic process - Gene Ontology Molecular Function:
- ATP binding
deoxyribonuclease activity
GTP binding
identical protein binding
magnesium ion binding
nucleoside diphosphate kinase activity
poly(A) RNA binding
ribosomal small subunit binding
RNA polymerase II regulatory region sequence-specific DNA binding
single-stranded DNA binding - Gene Ontology Cellular Component:
- centrosome
cytoplasm
cytosol
extracellular exosome
intermediate filament
membrane
mitochondrial outer membrane
myelin sheath
nucleus
perinuclear region of cytoplasm
ruffle membrane - Keywords:
- 3D-structure
ATP-binding
Acetylation
Alternative splicing
Complete proteome
Cytoplasm
Differentiation
Direct protein sequencing
Endocytosis
Isopeptide bond
Kinase
Magnesium
Metal-binding
Neurogenesis
Nucleotide metabolism
Nucleotide-binding
Nucleus
Polymorphism
Reference proteome
Transferase
Ubl conjugation - Interacts With:
- Itself; Q61097; P10911; P22392; Q13232; O00746; Q7Z2X4; O15160; Q86TP1; Q8N9Q2; Q9Y3F4; O14787; Q96HA8