Names & Taxonomy

Uniprot ID:
P07814
Entry Name:
SYEP_HUMAN
Status:
reviewed
Protein Names:
Bifunctional glutamate/proline--tRNA ligase (Bifunctional aminoacyl-tRNA synthetase) (Cell proliferation-inducing gene 32 protein) (Glutamatyl-prolyl-tRNA synthetase) [Includes: Glutamate--tRNA ligase (EC 6.1.1.17) (Glutamyl-tRNA synthetase) (GluRS); Proline--tRNA ligase (EC 6.1.1.15) (Prolyl-tRNA synthetase)]
Gene Names:
EPRS GLNS PARS QARS QPRS PIG32
Gene Names Orf:
PIG32
Gene Names Primary:
EPRS
Organism:
Homo sapiens (Human)

Structure

Length:
1512
Sequence:
MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm

Function

Function:
Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Catalytic Activity:
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).; ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).
Cross Reference Drug Bank:
DB00172
Gene Ontology Go:
aminoacyl-tRNA synthetase multienzyme complex
cytoplasm
cytosol
GAIT complex
intracellular ribonucleoprotein complex
membrane
ATP binding
glutamate-tRNA ligase activity
GTPase binding
proline-tRNA ligase activity
RNA stem-loop binding
cellular nitrogen compound metabolic process
cellular response to interferon-gamma
gene expression
glutamyl-tRNA aminoacylation
negative regulation of translation
prolyl-tRNA aminoacylation
protein complex assembly
selenium compound metabolic process
small molecule metabolic process
tRNA aminoacylation for protein translation
tRNA modification
tRNA processing
Gene Ontology Biological Process:
cellular nitrogen compound metabolic process
cellular response to interferon-gamma
gene expression
glutamyl-tRNA aminoacylation
negative regulation of translation
prolyl-tRNA aminoacylation
protein complex assembly
selenium compound metabolic process
small molecule metabolic process
tRNA aminoacylation for protein translation
tRNA modification
tRNA processing
Gene Ontology Molecular Function:
ATP binding
glutamate-tRNA ligase activity
GTPase binding
proline-tRNA ligase activity
RNA stem-loop binding
Gene Ontology Cellular Component:
aminoacyl-tRNA synthetase multienzyme complex
cytoplasm
cytosol
GAIT complex
intracellular ribonucleoprotein complex
membrane
Keywords:
3D-structure
ATP-binding
Acetylation
Aminoacyl-tRNA synthetase
Complete proteome
Cytoplasm
Ligase
Multifunctional enzyme
Nucleotide-binding
Phosphoprotein
Polymorphism
Protein biosynthesis
RNA-binding
Reference proteome
Repeat
Translation regulation
Interacts With:
P41252

Publication

PubMed ID:
17974005 16710414 15489334 1988429 3290852 1756734 1556743 15479637 15994936 17081983 16964243 18220336 18691976 18669648 19413330 19647514 19690332 19608861 20068231 21269460 21908771 21220307 21406692 23071094 24275569 25944712 11123902