Names & Taxonomy
- Uniprot ID:
- P07814
- Entry Name:
- SYEP_HUMAN
- Status:
- reviewed
- Protein Names:
- Bifunctional glutamate/proline--tRNA ligase (Bifunctional aminoacyl-tRNA synthetase) (Cell proliferation-inducing gene 32 protein) (Glutamatyl-prolyl-tRNA synthetase) [Includes: Glutamate--tRNA ligase (EC 6.1.1.17) (Glutamyl-tRNA synthetase) (GluRS); Proline--tRNA ligase (EC 6.1.1.15) (Prolyl-tRNA synthetase)]
- Gene Names:
- EPRS GLNS PARS QARS QPRS PIG32
- Gene Names Orf:
- PIG32
- Gene Names Primary:
- EPRS
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1512
- Sequence:
- MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm
Function
- Function:
- Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
- Catalytic Activity:
- ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).; ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).
- Cross Reference Drug Bank:
- DB00172
- Gene Ontology Go:
- aminoacyl-tRNA synthetase multienzyme complex
cytoplasm
cytosol
GAIT complex
intracellular ribonucleoprotein complex
membrane
ATP binding
glutamate-tRNA ligase activity
GTPase binding
proline-tRNA ligase activity
RNA stem-loop binding
cellular nitrogen compound metabolic process
cellular response to interferon-gamma
gene expression
glutamyl-tRNA aminoacylation
negative regulation of translation
prolyl-tRNA aminoacylation
protein complex assembly
selenium compound metabolic process
small molecule metabolic process
tRNA aminoacylation for protein translation
tRNA modification
tRNA processing - Gene Ontology Biological Process:
- cellular nitrogen compound metabolic process
cellular response to interferon-gamma
gene expression
glutamyl-tRNA aminoacylation
negative regulation of translation
prolyl-tRNA aminoacylation
protein complex assembly
selenium compound metabolic process
small molecule metabolic process
tRNA aminoacylation for protein translation
tRNA modification
tRNA processing - Gene Ontology Molecular Function:
- ATP binding
glutamate-tRNA ligase activity
GTPase binding
proline-tRNA ligase activity
RNA stem-loop binding - Gene Ontology Cellular Component:
- aminoacyl-tRNA synthetase multienzyme complex
cytoplasm
cytosol
GAIT complex
intracellular ribonucleoprotein complex
membrane - Keywords:
- 3D-structure
ATP-binding
Acetylation
Aminoacyl-tRNA synthetase
Complete proteome
Cytoplasm
Ligase
Multifunctional enzyme
Nucleotide-binding
Phosphoprotein
Polymorphism
Protein biosynthesis
RNA-binding
Reference proteome
Repeat
Translation regulation - Interacts With:
- P41252