Names & Taxonomy
- Uniprot ID:
- P06746
- Entry Name:
- DPOLB_HUMAN
- Status:
- reviewed
- Protein Names:
- DNA polymerase beta (EC 2.7.7.7) (EC 4.2.99.-)
- Gene Names:
- POLB
- Gene Names Primary:
- POLB
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 335
- Sequence:
- MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus. Cytoplasm. Note=Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
Function
- Function:
- Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
- Catalytic Activity:
- Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Active Site:
- ACT_SITE 72 72 Schiff-base intermediate with DNA.
- Cross Reference Drug Bank:
- DB00987
- Gene Ontology Go:
- cytoplasm
microtubule
nucleoplasm
nucleus
protein complex
spindle microtubule
damaged DNA binding
DNA-directed DNA polymerase activity
enzyme binding
lyase activity
metal ion binding
microtubule binding
aging
base-excision repair
base-excision repair, gap-filling
cellular response to DNA damage stimulus
DNA repair
DNA-dependent DNA replication
homeostasis of number of cells
immunoglobulin heavy chain V-D-J recombination
inflammatory response
intrinsic apoptotic signaling pathway in response to DNA damage
lymph node development
neuron apoptotic process
pyrimidine dimer repair
response to ethanol
response to gamma radiation
response to hyperoxia
salivary gland morphogenesis
somatic hypermutation of immunoglobulin genes
spleen development - Gene Ontology Biological Process:
- aging
base-excision repair
base-excision repair, gap-filling
cellular response to DNA damage stimulus
DNA-dependent DNA replication
DNA repair
homeostasis of number of cells
immunoglobulin heavy chain V-D-J recombination
inflammatory response
intrinsic apoptotic signaling pathway in response to DNA damage
lymph node development
neuron apoptotic process
pyrimidine dimer repair
response to ethanol
response to gamma radiation
response to hyperoxia
salivary gland morphogenesis
somatic hypermutation of immunoglobulin genes
spleen development - Gene Ontology Molecular Function:
- damaged DNA binding
DNA-directed DNA polymerase activity
enzyme binding
lyase activity
metal ion binding
microtubule binding - Gene Ontology Cellular Component:
- cytoplasm
microtubule
nucleoplasm
nucleus
protein complex
spindle microtubule - Keywords:
- 3D-structure
Acetylation
Complete proteome
Cytoplasm
DNA damage
DNA repair
DNA replication
DNA synthesis
DNA-binding
DNA-directed DNA polymerase
Isopeptide bond
Lyase
Magnesium
Metal-binding
Methylation
Nucleotidyltransferase
Nucleus
Polymorphism
Reference proteome
Sodium
Transferase
Ubl conjugation - Interacts With:
- Q9H5J8; P29144