Names & Taxonomy
- Uniprot ID:
- P00374
- Entry Name:
- DYR_HUMAN
- Status:
- reviewed
- Protein Names:
- Dihydrofolate reductase (EC 1.5.1.3)
- Gene Names:
- DHFR
- Gene Names Primary:
- DHFR
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 187
- Sequence:
- MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
- Proteomes:
- UP000005640
Function
- Function:
- Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
- Pathway:
- Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
- Catalytic Activity:
- 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.7 uM for dihydrofolate
- Cross Reference Drug Bank:
- DB00798 DB00563 DB00642 DB06813 DB01131 DB00205 DB00440 DB01157
- Gene Ontology Go:
- cytosol
nucleoplasm
dihydrofolate reductase activity
drug binding
folic acid binding
methotrexate binding
mRNA binding
NADPH binding
axon regeneration
dihydrofolate metabolic process
folic acid metabolic process
G1/S transition of mitotic cell cycle
glycine biosynthetic process
mitotic cell cycle
nitric oxide metabolic process
nucleotide biosynthetic process
one-carbon metabolic process
oxidation-reduction process
positive regulation of nitric-oxide synthase activity
regulation of nitric-oxide synthase activity
regulation of removal of superoxide radicals
regulation of transcription involved in G1/S transition of mitotic cell cycle
response to methotrexate
small molecule metabolic process
tetrahydrobiopterin biosynthetic process
tetrahydrofolate biosynthetic process
tetrahydrofolate metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process - Gene Ontology Biological Process:
- axon regeneration
dihydrofolate metabolic process
folic acid metabolic process
G1/S transition of mitotic cell cycle
glycine biosynthetic process
mitotic cell cycle
nitric oxide metabolic process
nucleotide biosynthetic process
one-carbon metabolic process
oxidation-reduction process
positive regulation of nitric-oxide synthase activity
regulation of nitric-oxide synthase activity
regulation of removal of superoxide radicals
regulation of transcription involved in G1/S transition of mitotic cell cycle
response to methotrexate
small molecule metabolic process
tetrahydrobiopterin biosynthetic process
tetrahydrofolate biosynthetic process
tetrahydrofolate metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process - Gene Ontology Molecular Function:
- dihydrofolate reductase activity
drug binding
folic acid binding
methotrexate binding
mRNA binding
NADPH binding - Gene Ontology Cellular Component:
- cytosol
nucleoplasm - Keywords:
- 3D-structure
Alternative splicing
Complete proteome
Disease mutation
Methotrexate resistance
NADP
One-carbon metabolism
Oxidoreductase
RNA-binding
Reference proteome