Names & Taxonomy

Uniprot ID:
O95237
Entry Name:
LRAT_HUMAN
Status:
reviewed
Protein Names:
Lecithin retinol acyltransferase (EC 2.3.1.135) (Phosphatidylcholine--retinol O-acyltransferase)
Gene Names:
LRAT
Gene Names Primary:
LRAT
Organism:
Homo sapiens (Human)

Structure

Length:
230
Sequence:
MKNPMLEVVSLLLEKLLLISNFTLFSSGAAGEDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRSVLASAVLGLASIVCTGLVSYTTLPAIFIPFFLWMAG
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Endoplasmic reticulum membrane

Function

Function:
Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments.
Pathway:
Cofactor metabolism; retinol metabolism.
Catalytic Activity:
Phosphatidylcholine + retinol-- = 2-acylglycerophosphocholine + retinyl-ester--.
Enzyme Regulation:
ENZYME REGULATION: Inhibited by all-trans-retinyl alpha-bromoacetate and N-boc-L-biocytinyl-11-aminoundecane chloro-methyl ketone (BACMK).
Active Site:
ACT_SITE 161 161 Acyl-thioester intermediate.
Cross Reference Drug Bank:
DB00162
Gene Ontology Go:
endoplasmic reticulum membrane
integral component of membrane
multivesicular body
perinuclear region of cytoplasm
rough endoplasmic reticulum
phosphatidylcholine-retinol O-acyltransferase activity
retinoic acid binding
retinol binding
transferase activity, transferring acyl groups
fat-soluble vitamin metabolic process
phototransduction, visible light
positive regulation of lipid transport
retinoic acid metabolic process
retinoid metabolic process
retinol metabolic process
small molecule metabolic process
visual perception
vitamin A metabolic process
vitamin metabolic process
Gene Ontology Biological Process:
fat-soluble vitamin metabolic process
phototransduction, visible light
positive regulation of lipid transport
retinoic acid metabolic process
retinoid metabolic process
retinol metabolic process
small molecule metabolic process
visual perception
vitamin A metabolic process
vitamin metabolic process
Gene Ontology Molecular Function:
phosphatidylcholine-retinol O-acyltransferase activity
retinoic acid binding
retinol binding
transferase activity, transferring acyl groups
Gene Ontology Cellular Component:
endoplasmic reticulum membrane
integral component of membrane
multivesicular body
perinuclear region of cytoplasm
rough endoplasmic reticulum
Keywords:
Acyltransferase
Complete proteome
Cytoplasm
Disease mutation
Endoplasmic reticulum
Endosome
Leber congenital amaurosis
Membrane
Polymorphism
Reference proteome
Sensory transduction
Transferase
Transmembrane
Transmembrane helix
Vision

Publication

PubMed ID:
9920938 15474300 14702039 15489334 10819989 18055821 18544127 11381255 17011878