Names & Taxonomy

Uniprot ID:
O94925
Entry Name:
GLSK_HUMAN
Status:
reviewed
Protein Names:
Glutaminase kidney isoform, mitochondrial (GLS) (EC 3.5.1.2) (K-glutaminase) (L-glutamine amidohydrolase)
Gene Names:
GLS GLS1 KIAA0838
Gene Names Primary:
GLS
Organism:
Homo sapiens (Human)

Structure

Length:
669
Sequence:
MMRLRGSGMLRDLLLRSPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLHPWWGGGGWPAEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAPAAPGPKDGPGETDAFGNSEGKELVASGENKIKQGLLPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSTGDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLIKQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Isoform 1: Cytoplasm, cytosol.; Isoform 3: Mitochondrion.

Function

Function:
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Catalytic Activity:
L-glutamine + H(2)O = L-glutamate + NH(3).
Kinetics:
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.9 mM for glutamine (isoform 1)
Enzyme Regulation:
ENZYME REGULATION: Isoform 1 and isoform 3 are activated by phosphate. Inhibited by BPTES. BPTES binds between subunits and favors dissociation of the tetramer into dimers.
Cross Reference Drug Bank:
DB00130
Gene Ontology Go:
cytosol
mitochondrial matrix
mitochondrion
presynapse
glutaminase activity
cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
gene expression
glutamate biosynthetic process
glutamate secretion
glutamine catabolic process
neurotransmitter secretion
protein homotetramerization
regulation of respiratory gaseous exchange by neurological system process
small molecule metabolic process
suckling behavior
synaptic transmission
transcription initiation from RNA polymerase II promoter
Gene Ontology Biological Process:
cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
gene expression
glutamate biosynthetic process
glutamate secretion
glutamine catabolic process
neurotransmitter secretion
protein homotetramerization
regulation of respiratory gaseous exchange by neurological system process
small molecule metabolic process
suckling behavior
synaptic transmission
transcription initiation from RNA polymerase II promoter
Gene Ontology Molecular Function:
glutaminase activity
Gene Ontology Cellular Component:
cytosol
mitochondrial matrix
mitochondrion
presynapse
Keywords:
3D-structure
ANK repeat
Acetylation
Alternative splicing
Complete proteome
Cytoplasm
Direct protein sequencing
Hydrolase
Mitochondrion
Phosphoprotein
Polymorphism
Reference proteome
Repeat
Transit peptide

Publication

PubMed ID:
11015561 10048485 10719215 15489334 14759258 16899818 17940881 19139490 19608861 21269460 22228304 24275569 25944712 22049910