Names & Taxonomy
- Uniprot ID:
- O94760
- Entry Name:
- DDAH1_HUMAN
- Status:
- reviewed
- Protein Names:
- N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (DDAH-1) (Dimethylarginine dimethylaminohydrolase 1) (EC 3.5.3.18) (DDAHI) (Dimethylargininase-1)
- Gene Names:
- DDAH1 DDAH
- Gene Names Primary:
- DDAH1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 285
- Sequence:
- MAGLGHPAAFGRATHAVVRALPESLGQHALRSAKGEEVDVARAERQHQLYVGVLGSKLGLQVVELPADESLPDCVFVEDVAVVCEETALITRPGAPSRRKEVDMMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLTCCSVLINKKVDS
- Proteomes:
- UP000005640
Function
- Function:
- Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
- Catalytic Activity:
- N(omega),N(omega)-dimethyl-L-arginine + H(2)O = dimethylamine + L-citrulline.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=69 uM for asymmetric dimethylarginine (ADMA)
- Enzyme Regulation:
- ENZYME REGULATION: Inhibited by zinc ions (By similarity). Enzyme purified in the absence of 1,10-phenanthroline contains on average 0.4 zinc atoms per subunit. Inhibited by 4-hydroxy-nonenal through the formation of a covalent adduct with His-173. Competitively inhibited by N(5)-iminopropyl-ornithine.
- Active Site:
- ACT_SITE 173 173 Proton donor.
- Cross Reference Drug Bank:
- DB00155
- Gene Ontology Go:
- cytosol
extracellular exosome
mitochondrion
amino acid binding
catalytic activity
dimethylargininase activity
metal ion binding
arginine catabolic process
citrulline metabolic process
nitric oxide mediated signal transduction
nitric oxide metabolic process
positive regulation of angiogenesis
positive regulation of nitric oxide biosynthetic process
regulation of nitric-oxide synthase activity
regulation of systemic arterial blood pressure
small molecule metabolic process - Gene Ontology Biological Process:
- arginine catabolic process
citrulline metabolic process
nitric oxide mediated signal transduction
nitric oxide metabolic process
positive regulation of angiogenesis
positive regulation of nitric oxide biosynthetic process
regulation of nitric-oxide synthase activity
regulation of systemic arterial blood pressure
small molecule metabolic process - Gene Ontology Molecular Function:
- amino acid binding
catalytic activity
dimethylargininase activity
metal ion binding - Gene Ontology Cellular Component:
- cytosol
extracellular exosome
mitochondrion - Keywords:
- 3D-structure
Acetylation
Alternative splicing
Complete proteome
Direct protein sequencing
Hydrolase
Metal-binding
Reference proteome
S-nitrosylation
Zinc