Names & Taxonomy

Uniprot ID:
O76074
Entry Name:
PDE5A_HUMAN
Status:
reviewed
Protein Names:
cGMP-specific 3',5'-cyclic phosphodiesterase (EC 3.1.4.35) (cGMP-binding cGMP-specific phosphodiesterase) (CGB-PDE)
Gene Names:
PDE5A PDE5
Gene Names Primary:
PDE5A
Organism:
Homo sapiens (Human)

Structure

Length:
875
Sequence:
MERAGPSFGQQRQQQQPQQQKQQQRDQDSVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREHDANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN
Proteomes:
UP000005640

Function

Function:
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP (PubMed:9714779, PubMed:15489334). Specifically regulates nitric-oxide-generated cGMP (PubMed:15489334).
Pathway:
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
Catalytic Activity:
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate.
Cofactor:
COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Enzyme Regulation:
ENZYME REGULATION: Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A and is effective in the treatment of penile erectile dysfunction. Also inhibited by zaprinast.
Active Site:
ACT_SITE 613 613 Proton donor.
Cross Reference Drug Bank:
DB06237 DB00201 DB00975 DB00806 DB00203 DB00820 DB00277 DB06267 DB00862
Gene Ontology Go:
cytosol
3',5'-cyclic-GMP phosphodiesterase activity
3',5'-cyclic-nucleotide phosphodiesterase activity
cGMP binding
metal ion binding
blood coagulation
cGMP catabolic process
negative regulation of cardiac muscle contraction
negative regulation of T cell proliferation
positive regulation of cardiac muscle hypertrophy
positive regulation of MAP kinase activity
positive regulation of oocyte development
regulation of cGMP metabolic process
relaxation of cardiac muscle
signal transduction
Gene Ontology Biological Process:
blood coagulation
cGMP catabolic process
negative regulation of cardiac muscle contraction
negative regulation of T cell proliferation
positive regulation of cardiac muscle hypertrophy
positive regulation of MAP kinase activity
positive regulation of oocyte development
regulation of cGMP metabolic process
relaxation of cardiac muscle
signal transduction
Gene Ontology Molecular Function:
3',5'-cyclic-GMP phosphodiesterase activity
3',5'-cyclic-nucleotide phosphodiesterase activity
cGMP binding
metal ion binding
Gene Ontology Cellular Component:
cytosol
Keywords:
3D-structure
Allosteric enzyme
Alternative splicing
Complete proteome
Hydrolase
Metal-binding
Nucleotide-binding
Phosphoprotein
Polymorphism
Reference proteome
Repeat
cGMP
cGMP-binding

Publication

PubMed ID:
9714779 9716380 9642111 14702039 15815621 15489334 10393044 11723116 12955149 25799991