Names & Taxonomy
- Uniprot ID:
- O14802
- Entry Name:
- RPC1_HUMAN
- Status:
- reviewed
- Protein Names:
- DNA-directed RNA polymerase III subunit RPC1 (RNA polymerase III subunit C1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit) (DNA-directed RNA polymerase III subunit A) (RNA polymerase III 155 kDa subunit) (RPC155) (RNA polymerase III subunit C160)
- Gene Names:
- POLR3A
- Gene Names Primary:
- POLR3A
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1390
- Sequence:
- MVKEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNQHAPLLYGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKKQFLDYLKRPGLTYLQKRGLKKKISDKCRKKNICHHCGAFNGTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETAIEHNKEVEPLLGRAQENLNPLVVLNLFKRIPAEDVPLLLMNPEAGKPSDLILTRLLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEIIFLNDVIKKHRISGAKTQMIMEDWDFLQLQCALYINSELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVQNGPEVHPGANFIQQRHTQMKRFLKYGNREKMAQELKYGDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARVKPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANLRTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVIRDHAGSACLRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPAAMEGKDEPLEFKRVLDNIKAVFPCPSEPALSKNELILTTESIMKKSEFLCCQDSFLQEIKKFIKGVSEKIKKTRDKYGINDNGTTEPRVLYQLDRITPTQVEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTLLGEISEYIEEVFLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDVAVHGEAVVCVTPRENSKSSMYYVLQFLKEDLPKVVVQGIPEVSRAVIHIDEQSGKEKYKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRPLIFDTNEFHIPLVT
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus.
Function
- Function:
- DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
- Catalytic Activity:
- Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
- Gene Ontology Go:
- cytosol
DNA-directed RNA polymerase III complex
membrane
nucleoplasm
chromatin binding
DNA binding
DNA-directed RNA polymerase activity
metal ion binding
defense response to virus
gene expression
innate immune response
positive regulation of interferon-beta production
positive regulation of protein targeting to mitochondrion
positive regulation of type I interferon production
regulation of mitophagy
termination of RNA polymerase III transcription
transcription elongation from RNA polymerase III promoter
transcription from RNA polymerase III promoter
transcription, DNA-templated - Gene Ontology Biological Process:
- defense response to virus
gene expression
innate immune response
positive regulation of interferon-beta production
positive regulation of protein targeting to mitochondrion
positive regulation of type I interferon production
regulation of mitophagy
termination of RNA polymerase III transcription
transcription, DNA-templated
transcription elongation from RNA polymerase III promoter
transcription from RNA polymerase III promoter - Gene Ontology Molecular Function:
- chromatin binding
DNA binding
DNA-directed RNA polymerase activity
metal ion binding - Gene Ontology Cellular Component:
- cytosol
DNA-directed RNA polymerase III complex
membrane
nucleoplasm - Keywords:
- Acetylation
Antiviral defense
Complete proteome
DNA-directed RNA polymerase
Disease mutation
Immunity
Innate immunity
Leukodystrophy
Magnesium
Metal-binding
Nucleotidyltransferase
Nucleus
Polymorphism
Reference proteome
Transcription
Transferase
Zinc