Names & Taxonomy
- Uniprot ID:
- O00267
- Entry Name:
- SPT5H_HUMAN
- Status:
- reviewed
- Protein Names:
- Transcription elongation factor SPT5 (hSPT5) (DRB sensitivity-inducing factor 160 kDa subunit) (DSIF p160) (DRB sensitivity-inducing factor large subunit) (DSIF large subunit) (Tat-cotransactivator 1 protein) (Tat-CT1 protein)
- Gene Names:
- SUPT5H SPT5 SPT5H
- Gene Names Primary:
- SUPT5H
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1087
- Sequence:
- MSDSEDSNFSEEEDSERSSDGEEAEVDEERRSAAGSEKEEEPEDEEEEEEEEEYDEEEEEEDDDRPPKKPRHGGFILDEADVDDEYEDEDQWEDGAEDILEKEEIEASNIDNVVLDEDRSGARRLQNLWRDQREEELGEYYMKKYAKSSVGETVYGGSDELSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPLQIKSVVAPEHVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKSWVRLKRGIYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKARMSLKDWFAKRKKFKRPPQRLFDAEKIRSLGGDVASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTESTGKEREHNFQPGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMGDHVKVIAGRFEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELVQLDPQTVGVIVRLERETFQVLNMYGKVVTVRHQAVTRKKDNRFAVALDSEQNNIHVKDIVKVIDGPHSGREGEIRHLFRSFAFLHCKKLVENGGMFVCKTRHLVLAGGSKPRDVTNFTVGGFAPMSPRISSPMHPSAGGQRGGFGSPGGGSGGMSRGRGRRDNELIGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISVDRQRLTTVGSRRPGGMTSTYGRTPMYGSQTPMYGSGSRTPMYGSQTPLQDGSRTPHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAFDDEPTPSPQAYGGTPNPQTPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQGSYQPSPSPQSYHQVAPSPAGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWVTTDIQVKVRDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDREATGVLLSIDGEDGIVRMDLDEQLKILNLRFLGKLLEA
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus
Function
- Function:
- Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
- Gene Ontology Go:
- DSIF complex
nucleoplasm
nucleus
enzyme binding
poly(A) RNA binding
protein heterodimerization activity
7-methylguanosine mRNA capping
cell cycle
chromatin remodeling
DNA-templated transcription, elongation
gene expression
negative regulation of DNA-templated transcription, elongation
negative regulation of mRNA polyadenylation
negative regulation of transcription from RNA polymerase II promoter
positive regulation of DNA-templated transcription, elongation
positive regulation of macroautophagy
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
response to organic substance
single stranded viral RNA replication via double stranded DNA intermediate
transcription elongation from RNA polymerase II promoter
transcription from RNA polymerase II promoter
viral process - Gene Ontology Biological Process:
- 7-methylguanosine mRNA capping
cell cycle
chromatin remodeling
DNA-templated transcription, elongation
gene expression
negative regulation of DNA-templated transcription, elongation
negative regulation of mRNA polyadenylation
negative regulation of transcription from RNA polymerase II promoter
positive regulation of DNA-templated transcription, elongation
positive regulation of macroautophagy
positive regulation of transcription from RNA polymerase II promoter
positive regulation of viral transcription
response to organic substance
single stranded viral RNA replication via double stranded DNA intermediate
transcription elongation from RNA polymerase II promoter
transcription from RNA polymerase II promoter
viral process - Gene Ontology Molecular Function:
- enzyme binding
poly(A) RNA binding
protein heterodimerization activity - Gene Ontology Cellular Component:
- DSIF complex
nucleoplasm
nucleus - Keywords:
- 3D-structure
Acetylation
Activator
Alternative splicing
Complete proteome
Direct protein sequencing
Isopeptide bond
Methylation
Nucleus
Phosphoprotein
Reference proteome
Repeat
Repressor
Transcription
Transcription regulation
Ubl conjugation - Interacts With:
- P24928; P62937; P63272; P54274
Publication
- PubMed ID:
- 8975720 9199507 9450929 9514752 15489334 9857195 10199401 10393184 10421630 10075709 10454543 10912001 10757782 11145967 11112772 11553615 11575923 11809800 11940650 12653964 12718890 12612062 15380072 15564463 14701750 15060154 15136722 16214896 17081983 16327805 17924679 18669648 19413330 20068231 21269460 24275569 25218447 25755297 19860741