Names & Taxonomy

Uniprot ID:
Q9Y6D5
Entry Name:
BIG2_HUMAN
Status:
reviewed
Protein Names:
Brefeldin A-inhibited guanine nucleotide-exchange protein 2 (Brefeldin A-inhibited GEP 2) (ADP-ribosylation factor guanine nucleotide-exchange factor 2)
Gene Names:
ARFGEF2 ARFGEP2 BIG2
Gene Names Primary:
ARFGEF2
Organism:
Homo sapiens (Human)

Structure

Length:
1785
Sequence:
MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICSCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTTPEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEAAEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAARFSHVLQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFINAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERLTDQEIGDGKGLDMARRCSVTSMESTVSSGTQTTVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSARFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKELTIATKSTKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMAIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTCHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQLSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPEVWDETCNCMLDIFKTTIPHVLLTWRPVGMEEDSSEKHLDVDLDRQSLSSIDKNPSERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETEDQGMYKYMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWEEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPAALSPVW
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm. Membrane. Golgi apparatus. Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network

Function

Function:
Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extend on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.
Enzyme Regulation:
ENZYME REGULATION: Inhibited by brefeldin A.
Gene Ontology Go:
asymmetric synapse
axonemal microtubule
cell junction
cytoplasmic vesicle
cytoplasmic, membrane-bounded vesicle
cytosol
dendritic spine
Golgi membrane
membrane
microtubule organizing center
perinuclear region of cytoplasm
recycling endosome
symmetric synapse
trans-Golgi network
ARF guanyl-nucleotide exchange factor activity
GABA receptor binding
guanyl-nucleotide exchange factor activity
protein kinase A regulatory subunit binding
endomembrane system organization
endosome organization
exocytosis
Golgi to plasma membrane transport
intracellular signal transduction
positive regulation of GTPase activity
positive regulation of tumor necrosis factor production
protein transport
receptor recycling
regulation of ARF protein signal transduction
Gene Ontology Biological Process:
endomembrane system organization
endosome organization
exocytosis
Golgi to plasma membrane transport
intracellular signal transduction
positive regulation of GTPase activity
positive regulation of tumor necrosis factor production
protein transport
receptor recycling
regulation of ARF protein signal transduction
Gene Ontology Molecular Function:
ARF guanyl-nucleotide exchange factor activity
GABA receptor binding
guanyl-nucleotide exchange factor activity
protein kinase A regulatory subunit binding
Gene Ontology Cellular Component:
asymmetric synapse
axonemal microtubule
cell junction
cytoplasmic, membrane-bounded vesicle
cytoplasmic vesicle
cytosol
dendritic spine
Golgi membrane
membrane
microtubule organizing center
perinuclear region of cytoplasm
recycling endosome
symmetric synapse
trans-Golgi network
Keywords:
3D-structure
Acetylation
Cell junction
Cell projection
Complete proteome
Cytoplasm
Cytoplasmic vesicle
Cytoskeleton
Disease mutation
Endosome
Golgi apparatus
Guanine-nucleotide releasing factor
Membrane
Phosphoprotein
Polymorphism
Protein transport
Reference proteome
Synapse
Transport
Interacts With:
Q9Y6D6; Q9UPT5-1; Q99417

Publication

PubMed ID:
10212200 11780052 10716990 12051703 12571360 15385626 15705715 17081983 16866877 16477018 17276987 17640864 17360629 18625701 18691976 18669648 19413330 19369195 19332778 19690332 20360857 20068231 21269460 21406692 22814378 24275569 14647276 16959974 23033978