Names & Taxonomy
- Uniprot ID:
- Q9Y3I0
- Entry Name:
- RTCB_HUMAN
- Status:
- reviewed
- Protein Names:
- tRNA-splicing ligase RtcB homolog (EC 6.5.1.3)
- Gene Names:
- RTCB C22orf28 HSPC117
- Gene Names Orf:
- HSPC117
- Gene Names Primary:
- RTCB
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 505
- Sequence:
- MSRSYNDELQFLEKINKNCWRIKKGFVPNMQVEGVFYVNDALEKLMFEELRNACRGGGVGGFLPAMKQIGNVAALPGIVHRSIGLPDVHSGYGFAIGNMAAFDMNDPEAVVSPGGVGFDINCGVRLLRTNLDESDVQPVKEQLAQAMFDHIPVGVGSKGVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSARAKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDLDLHVIYDVSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGISKKAIKLRPIAVIKG
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus
Function
- Function:
- Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
- Catalytic Activity:
- ATP + (ribonucleotide)(n) + (ribonucleotide)(m) = AMP + diphosphate + (ribonucleotide)(n+m).
- Cofactor:
- COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
- Active Site:
- ACT_SITE 428 428 GMP-histidine intermediate.
- Gene Ontology Go:
- cytoplasm
endoplasmic reticulum membrane
nuclear envelope
nucleoplasm
nucleus
tRNA-splicing ligase complex
ATP binding
metal ion binding
poly(A) RNA binding
RNA ligase (ATP) activity
vinculin binding
gene expression
in utero embryonic development
placenta development
tRNA processing
tRNA splicing, via endonucleolytic cleavage and ligation - Gene Ontology Biological Process:
- gene expression
in utero embryonic development
placenta development
tRNA processing
tRNA splicing, via endonucleolytic cleavage and ligation - Gene Ontology Molecular Function:
- ATP binding
metal ion binding
poly(A) RNA binding
RNA ligase (ATP) activity
vinculin binding - Gene Ontology Cellular Component:
- cytoplasm
endoplasmic reticulum membrane
nuclear envelope
nucleoplasm
nucleus
tRNA-splicing ligase complex - Keywords:
- ATP-binding
Complete proteome
Cytoplasm
Direct protein sequencing
Ligase
Manganese
Metal-binding
Nucleotide-binding
Nucleus
Polymorphism
Reference proteome
tRNA processing - Interacts With:
- Q9UKG1