Names & Taxonomy

Uniprot ID:
Q9UHY7
Entry Name:
ENOPH_HUMAN
Status:
reviewed
Protein Names:
Enolase-phosphatase E1 (EC 3.1.3.77) (2,3-diketo-5-methylthio-1-phosphopentane phosphatase) (MASA homolog)
Gene Names:
ENOPH1 MASA MSTP145
Gene Names Orf:
MSTP145
Gene Names Primary:
ENOPH1
Organism:
Homo sapiens (Human)

Structure

Length:
261
Sequence:
MVVLSVPAEVTVILLDIEGTTTPIAFVKDILFPYIEENVKEYLQTHWEEEECQQDVSLLRKQAEEDAHLDGAVPIPAASGNGVDDLQQMIQAVVDNVCWQMSLDRKTTALKQLQGHMWRAAFTAGRMKAEFFADVVPAVRKWREAGMKVYIYSSGSVEAQKLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFLTDVTREASAAEEADVHVAVVVRPGNAGLTDDEKTYYSLITSFSELYLPSST
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Cytoplasm

Function

Function:
Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
Pathway:
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.
Catalytic Activity:
5-(methylthio)-2,3-dioxopentyl phosphate + H(2)O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.
Cofactor:
COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; ; Note=Binds 1 Mg(2+) ion per subunit.;
Gene Ontology Go:
cytosol
extracellular exosome
nucleus
acireductone synthase activity
magnesium ion binding
cellular nitrogen compound metabolic process
dephosphorylation
L-methionine biosynthetic process from methylthioadenosine
polyamine metabolic process
small molecule metabolic process
sulfur amino acid metabolic process
Gene Ontology Biological Process:
cellular nitrogen compound metabolic process
dephosphorylation
L-methionine biosynthetic process from methylthioadenosine
polyamine metabolic process
small molecule metabolic process
sulfur amino acid metabolic process
Gene Ontology Molecular Function:
acireductone synthase activity
magnesium ion binding
Gene Ontology Cellular Component:
cytosol
extracellular exosome
nucleus
Keywords:
3D-structure
Alternative splicing
Amino-acid biosynthesis
Complete proteome
Cytoplasm
Hydrolase
Magnesium
Metal-binding
Methionine biosynthesis
Nucleus
Reference proteome

Publication

PubMed ID:
10931946 14702039 15489334 21269460 15843022