Names & Taxonomy

Uniprot ID:
Q9NW08
Entry Name:
RPC2_HUMAN
Status:
reviewed
Protein Names:
DNA-directed RNA polymerase III subunit RPC2 (RNA polymerase III subunit C2) (EC 2.7.7.6) (C128) (DNA-directed RNA polymerase III 127.6 kDa polypeptide) (DNA-directed RNA polymerase III subunit B)
Gene Names:
POLR3B
Gene Names Primary:
POLR3B
Organism:
Homo sapiens (Human)

Structure

Length:
1133
Sequence:
MDVLAEEFGNLTPEQLAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLKYLNIYVGLPDVEESFNVTRPVSPHECRLRDMTYSAPITVDIEYTRGSQRIIRNALPIGRMPIMLRSSNCVLTGKTPAEFAKLNECPLDPGGYFIVKGVEKVILIQEQLSKNRIIVEADRKGAVGASVTSSTHEKKSRTNMAVKQGRFYLRHNTLSEDIPIVIIFKAMGVESDQEIVQMIGTEEHVMAAFGPSLEECQKAQIFTQMQALKYIGNKVRRQRMWGGGPKKTKIEEARELLASTILTHVPVKEFNFRAKCIYTAVMVRRVILAQGDNKVDDRDYYGNKRLELAGQLLSLLFEDLFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGNWSLKRFKMDRQGVTQVLSRLSYISALGMMTRISSQFEKTRKVSGPRSLQPSQWGMLCPSDTPEGEACGLVKNLALMTHITTDMEDGPIVKLASNLGVEDVNLLCGEELSYPNVFLVFLNGNILGVIRDHKKLVNTFRLMRRAGYINEFVSISTNLTDRCVYISSDGGRLCRPYIIVKKQKPAVTNKHMEELAQGYRNFEDFLHESLVEYLDVNEENDCNIALYEHTINKDTTHLEIEPFTLLGVCAGLIPYPHHNQSPRNTYQCAMGKQAMGTIGYNQRNRIDTLMYLLAYPQKPMVKTKTIELIEFEKLPAGQNATVAVMSYSGYDIEDALVLNKASLDRGFGRCLVYKNAKCTLKRYTNQTFDKVMGPMLDAATRKPIWRHEILDADGICSPGEKVENKQVLVNKSMPTVTQIPLEGSNVPQQPQYKDVPITYKGATDSYIEKVMISSNAEDAFLIKMLLRQTRRPEIGDKFSSRHGQKGVCGLIVPQEDMPFCDSGICPDIIMNPHGFPSRMTVGKLIELLAGKAGVLDGRFHYGTAFGGSKVKDVCEDLVRHGYNYLGKDYVTSGITGEPLEAYIYFGPVYYQKLKHMVLDKMHARARGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIGYGASMLLLERLMISSDAFEVDVCGQCGLLGYSGWCHYCKSSCHVSSLRIPYACKLLFQELQSMNIIPRLKLSKYNE
Proteomes:
UP000005640

Subcellular location

Subcellular Location:
Nucleus

Function

Function:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Catalytic Activity:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Gene Ontology Go:
cytosol
DNA-directed RNA polymerase III complex
nucleoplasm
DNA binding
DNA-directed RNA polymerase activity
metal ion binding
ribonucleoside binding
defense response to virus
gene expression
innate immune response
positive regulation of innate immune response
positive regulation of interferon-beta production
positive regulation of type I interferon production
termination of RNA polymerase III transcription
transcription elongation from RNA polymerase III promoter
transcription from RNA polymerase III promoter
Gene Ontology Biological Process:
defense response to virus
gene expression
innate immune response
positive regulation of innate immune response
positive regulation of interferon-beta production
positive regulation of type I interferon production
termination of RNA polymerase III transcription
transcription elongation from RNA polymerase III promoter
transcription from RNA polymerase III promoter
Gene Ontology Molecular Function:
DNA binding
DNA-directed RNA polymerase activity
metal ion binding
ribonucleoside binding
Gene Ontology Cellular Component:
cytosol
DNA-directed RNA polymerase III complex
nucleoplasm
Keywords:
Alternative splicing
Antiviral defense
Complete proteome
DNA-directed RNA polymerase
Disease mutation
Immunity
Innate immunity
Leukodystrophy
Metal-binding
Nucleotidyltransferase
Nucleus
Polymorphism
Reference proteome
Transcription
Transferase
Zinc
Zinc-finger

Publication

PubMed ID:
12391170 14702039 16541075 15489334 19631370 19609254 21269460 22036171 22036172 23355746