Names & Taxonomy
- Uniprot ID:
- Q9NTG7
- Entry Name:
- SIR3_HUMAN
- Status:
- reviewed
- Protein Names:
- NAD-dependent protein deacetylase sirtuin-3, mitochondrial (hSIRT3) (EC 3.5.1.-) (Regulatory protein SIR2 homolog 3) (SIR2-like protein 3)
- Gene Names:
- SIRT3 SIR2L3
- Gene Names Primary:
- SIRT3
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 399
- Sequence:
- MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Mitochondrion matrix
Function
- Function:
- NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
- Catalytic Activity:
- NAD(+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.
- Cofactor:
- COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; ; Note=Binds 1 zinc ion per subunit.;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=600 uM for NAD
- Active Site:
- ACT_SITE 248 248 Proton acceptor.
- Gene Ontology Go:
- mitochondrial inner membrane
mitochondrial matrix
mitochondrion
NAD+ ADP-ribosyltransferase activity
NAD+ binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
zinc ion binding
aerobic respiration
mitochondrion organization
negative regulation of peptidyl-lysine acetylation
organelle organization
peptidyl-lysine deacetylation
protein ADP-ribosylation
protein deacetylation - Gene Ontology Biological Process:
- aerobic respiration
mitochondrion organization
negative regulation of peptidyl-lysine acetylation
organelle organization
peptidyl-lysine deacetylation
protein ADP-ribosylation
protein deacetylation - Gene Ontology Molecular Function:
- NAD+ ADP-ribosyltransferase activity
NAD+ binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
zinc ion binding - Gene Ontology Cellular Component:
- mitochondrial inner membrane
mitochondrial matrix
mitochondrion - Keywords:
- 3D-structure
Alternative splicing
Complete proteome
Hydrolase
Metal-binding
Mitochondrion
NAD
Polymorphism
Reference proteome
Transit peptide
Zinc - Interacts With:
- P25705; O60313; Q13309