Names & Taxonomy
- Uniprot ID:
- Q96C86
- Entry Name:
- DCPS_HUMAN
- Status:
- reviewed
- Protein Names:
- m7GpppX diphosphatase (EC 3.6.1.59) (DCS-1) (Decapping scavenger enzyme) (Hint-related 7meGMP-directed hydrolase) (Histidine triad nucleotide-binding protein 5) (Histidine triad protein member 5) (HINT-5) (Scavenger mRNA-decapping enzyme DcpS)
- Gene Names:
- DCPS DCS1 HINT5 HSPC015
- Gene Names Orf:
- HSPC015
- Gene Names Primary:
- DCPS
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 337
- Sequence:
- MADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASGDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEAQQS
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm. Nucleus. Note=Predominantly localized in the nucleus. Nucleocytoplasmic shuttling protein that can transiently enter the cytoplasm in mammalian cells in a XPO1/CRM1-dependent manner.
Function
- Function:
- Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP (PubMed:22985415). May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP (PubMed:14523240). Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death.
- Catalytic Activity:
- A 5'-(N(7)-methyl 5'-triphosphoguanosine)- + H(2)O = N(7)-methylguanosine 5'-phosphate + a 5'-diphospho-.
- Enzyme Regulation:
- ENZYME REGULATION: The hydrolytic product 7-methylguanosine diphosphate (m7GDP) efficiently inhibits the decapping scavenger activity and acts as a competitive inhibitor in vitro. Inhibited by 2,4-diaminoquinazoline.
- Active Site:
- ACT_SITE 277 277 Nucleophile.
- Gene Ontology Go:
- cytoplasm
cytosol
mitochondrion
nucleoplasm
nucleus
m7G(5')pppN diphosphatase activity
RNA 7-methylguanosine cap binding
cellular response to menadione
deadenylation-dependent decapping of nuclear-transcribed mRNA
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
gene expression
mRNA cis splicing, via spliceosome
negative regulation of programmed cell death
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay - Gene Ontology Biological Process:
- cellular response to menadione
deadenylation-dependent decapping of nuclear-transcribed mRNA
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
gene expression
mRNA cis splicing, via spliceosome
negative regulation of programmed cell death
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay - Gene Ontology Molecular Function:
- m7G(5')pppN diphosphatase activity
RNA 7-methylguanosine cap binding - Gene Ontology Cellular Component:
- cytoplasm
cytosol
mitochondrion
nucleoplasm
nucleus - Keywords:
- 3D-structure
Acetylation
Complete proteome
Cytoplasm
Direct protein sequencing
Disease mutation
Hydrolase
Mental retardation
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
mRNA processing
mRNA splicing