Names & Taxonomy
- Uniprot ID:
- Q96C36
- Entry Name:
- P5CR2_HUMAN
- Status:
- reviewed
- Protein Names:
- Pyrroline-5-carboxylate reductase 2 (P5C reductase 2) (P5CR 2) (EC 1.5.1.2)
- Gene Names:
- PYCR2
- Gene Names Primary:
- PYCR2
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 320
- Sequence:
- MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm
Function
- Function:
- Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH (PubMed:2722838, PubMed:6894153). Involved in cellular response to oxidative stress (PubMed:25865492).
- Pathway:
- Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
- Catalytic Activity:
- L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=0.64 mM for NADH
- Enzyme Regulation:
- ENZYME REGULATION: Subject to competitive inhibition by NADP. Not inhibited by proline.
- Cross Reference Drug Bank:
- DB00172
- Gene Ontology Go:
- mitochondrial matrix
mitochondrion
pyrroline-5-carboxylate reductase activity
cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
cellular response to oxidative stress
L-proline biosynthetic process
proline biosynthetic process
small molecule metabolic process - Gene Ontology Biological Process:
- cellular amino acid biosynthetic process
cellular nitrogen compound metabolic process
cellular response to oxidative stress
L-proline biosynthetic process
proline biosynthetic process
small molecule metabolic process - Gene Ontology Molecular Function:
- pyrroline-5-carboxylate reductase activity
- Gene Ontology Cellular Component:
- mitochondrial matrix
mitochondrion - Keywords:
- Acetylation
Amino-acid biosynthesis
Complete proteome
Cytoplasm
Direct protein sequencing
Disease mutation
Leukodystrophy
Mitochondrion
NADP
Oxidoreductase
Phosphoprotein
Proline biosynthesis
Reference proteome