Names & Taxonomy
- Uniprot ID:
- Q969G6
- Entry Name:
- RIFK_HUMAN
- Status:
- reviewed
- Protein Names:
- Riboflavin kinase (EC 2.7.1.26) (ATP:riboflavin 5'-phosphotransferase) (Flavokinase)
- Gene Names:
- RFK
- Gene Names Primary:
- RFK
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 155
- Sequence:
- MRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm
Function
- Function:
- Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
- Pathway:
- Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
- Catalytic Activity:
- ATP + riboflavin = ADP + FMN.
- Cofactor:
- COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Name=Mg(2+); Xref=ChEBI:CHEBI:18420; ; Note=Zinc or magnesium.;
- Active Site:
- ACT_SITE 79 79 Nucleophile.
- Cross Reference Drug Bank:
- DB00140
- Gene Ontology Go:
- cytoplasm
cytosol
mitochondrion
ATP binding
metal ion binding
riboflavin kinase activity
apoptotic process
FMN biosynthetic process
positive regulation of NAD(P)H oxidase activity
reactive oxygen species metabolic process
riboflavin biosynthetic process
riboflavin metabolic process
small molecule metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process - Gene Ontology Biological Process:
- apoptotic process
FMN biosynthetic process
positive regulation of NAD(P)H oxidase activity
reactive oxygen species metabolic process
riboflavin biosynthetic process
riboflavin metabolic process
small molecule metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process - Gene Ontology Molecular Function:
- ATP binding
metal ion binding
riboflavin kinase activity - Gene Ontology Cellular Component:
- cytoplasm
cytosol
mitochondrion - Keywords:
- 3D-structure
ATP-binding
Complete proteome
Cytoplasm
FMN
Flavoprotein
Kinase
Magnesium
Metal-binding
Nucleotide-binding
Reference proteome
Transferase
Zinc